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Jueves 2 de agosto 13.Hs. en el aula de seminarios del
LFBM<br><br>
<blockquote type=cite class=cite cite="">Titulo: Unraveling the
organization of GABA and glutamate axons in the dorsal raphe nucleus
using high-resolution anatomy<br><br>
Dr. Mariano Soiza Reilly<br><br>
Anesthesiology Dept., Boston Children’s Hospital, Harvard Medical School,
Boston, MA, USA<br><br>
Resumen: The dorsal raphe nucleus (DR) controls forebrain serotonin
(5-HT), regulating stress and emotional states. Dysregulation of DR
5-HT cells has been implicated in the pathophysiology of mood disorders
including depression and anxiety. DR neurons are regulated by
excitatory and inhibitory drives mediated by glutamate and GABA axons
arising from different brain regions. These multiple origins
suggest a very complex synaptic neuropil within the DR. However, we
do not know much about how these axons are organized or whether they
could be involved in any ordered synaptic arrangements, and this is in
part due to technical limitations. But now we can address these
questions using a recently developed high-resolution immunofluorescence
technique called Array Tomography. This technique allows the
quantitative analysis of the synaptic neuropil by studying multiple
synaptic antigens as well as their relationships to each other in the
same volume of tissue. Our study revealed new synaptic features of
DR circuits that may underlie to a direct GABAergic presynaptic
regulation of glutamate transmission, supporting a permissive role of
GABA on DR’s excitatory drive.<br><br>
Host: Urbano<br><br>
-- <br>
Francisco J. Urbano, PhD.<br>
IFIBYNE-CONICET<br>
Intendente Güiraldes 2160 <br>
Facultad Ciencias Exactas<br>
Pabellon 2- Piso 2<br>
Ciudad Universitaria<br>
(C1428EGA) Buenos Aires<br>
ARGENTINA<br>
Email:
<a href="mailto:fjurbano@fbmc.fcen.uba.ar">fjurbano@fbmc.fcen.uba.ar</a>
,<br>
<a href="mailto:fran.j.urbano@gmail.com">fran.j.urbano@gmail.com</a><br>
Phone: (+54)-11-4576 3368<br>
Fax: (+54)-11-4576 3321</blockquote></body>
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