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Jueves 9 de septiembre 13 Hs.<h3> Aula de seminarios del
LFBM</h3><blockquote type=cite class=cite cite="">Expositor: Alberto
Kornblihtt.<br>
Título: Organogénesis interespecífica<br><br>
Seminario sobre el paper recientemente publicado:<br><br>
<br>
<h2><b>Generation of Rat Pancreas in Mouse by Interspecific Blastocyst
Injection of Pluripotent Stem Cells</b></h2><br><br>
<br>
<a href="??">Toshihiro Kobayashi</a>,<a href="??">Tomoyuki
Yamaguchi</a>,<a href="??">Sanae Hamanaka</a>,<a href="??">Megumi
Kato-Itoh</a>,<a href="??">Yuji Yamazaki</a>,<a href="??">Makoto
Ibata</a>,<a href="??">Hideyuki Sato</a>,<a href="??">Youn-Su
Lee</a>,<a href="??">Jo-ichi Usui</a>,<a href="??">A.S.
Knisely</a>,<a href="??">Masumi Hirabayashi</a>,<a href="??">Hiromitsu
Nakauchi</a><br><br>
<br><br>
Interspecies Organogenesis <br>
The goal of regenerative medicine is to derive organs from patient’s
pluripotent stem cells. In a tour-de-force study, by injecting rat PSCs
into mouse blastocytes,
<a href="/abstract/S0092-8674(10)00843-3.htm">Nakauchi and colleagues</a>
generate a functioning rat pancreas in mice deficient for a pancreas; in
the process, the authors also describe the first viable rat-mouse
chimeras. <br><br>
Summary.<br>
The complexity of organogenesis hinders in vitro generation of organs
derived from a patient's pluripotent stem cells (PSCs), an ultimate goal
of regenerative medicine. Mouse wild-type PSCs injected into
<i>Pdx1</i><sup> / </sup>(pancreatogenesis-disabled) mouse blastocysts
developmentally compensated vacancy of the pancreatic “developmental
niche,” generating almost entirely PSC-derived pancreas. To examine the
potential for xenogenic approaches in blastocyst complementation, we
injected mouse or rat PSCs into rat or mouse blastocysts, respectively,
generating interspecific chimeras and thus confirming that PSCs can
contribute to xenogenic development between mouse and rat. The
development of these mouse/rat chimeras was primarily influenced by host
blastocyst and/or foster mother, evident by body size and
species-specific organogenesis. We further injected rat wild-type PSCs
into <i>Pdx1</i><sup> / </sup> mouse blastocysts, generating normally
functioning rat pancreas in <i>Pdx1</i><sup> / </sup> mice. These data
constitute proof of principle for interspecific blastocyst
complementation and for generation in vivo of organs derived from donor
PSCs using a xenogenic environment.<br><br>
-- <br>
Alejandro Colman-Lerner<br>
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