[Todos] Seminario IFBYNE Dr. S. Mongrand- Membrane rafts in plants, structure, evidence and role- Jueves 21 de julio 13 hs

[azelada en dna.uba.ar]

Aula Seminarios IFIBYNE

Jueves 21 de julio 13 hs.



Título: " Membrane rafts in plants, structure, evidence and role"

Dr. Sebastien Mongrand

Laboratorio de Biogénesis de membranas

Universidad de Bordeaux
Bordeaux, Francia



Key studies have contributed to reveal the structure, composition and
possible functions of membrane raft domains in plants. By immunogold
labeling, we recently evidenced that lateral segregation of lipids
(polyphosphoinositide (4,5) bisphosphate)  and proteins (REMORIN, the
first identified plant raft marker)  found enriched in Detergent-Insoluble
Membranes (DIM), indeed exists at the nanoscale level in plant plasma
membrane (PM), making  a strong correlation between DIM and rafts. « Omic
» analyses have revealed that a high proportion of proteins and lipids
associated with DIM may be involved in signalling pathways. Also our
recent data suggest that the PM lining plasmodesma (PD) may have similar
properties to rafts. Can rafts distribute between the different membrane
sub-domains? Quantitative proteomics reveals a dynamic association of
proteins to DIM upon elicitor signaling in tobacco cells. A role for plant
rafts as signal transduction platforms, similar to those documented for
mammalian cells, and in cell-to-cell communication through PD is starting
to emerge.






Mongrand S, Stanislas T, Bayer EM, Lherminier J, Simon-Plas F. Membrane rafts

in plant cells. Trends Plant Sci. 2010 Dec;15(12):656-63.Review.





Furt F, König S, Bessoule JJ, Sargueil F, Zallot R, Stanislas T, Noirot E,

Lherminier J, Simon-Plas F, Heilmann I, Mongrand S. Polyphosphoinositides are

enriched in plant membrane rafts and form microdomains in the plasma
membrane.

Plant Physiol. 2010 Apr;152(4):2173-87.



Lefebvre B, Timmers T, Mbengue M, Moreau S, Hervé C, Tóth K,
Bittencourt-Silvestre J, Klaus D, Deslandes L, Godiard L, Murray JD,
Udvardi MK, Raffaele S, Mongrand S, Cullimore J, Gamas P, Niebel A, Ott T.
A remorin protein interacts with symbiotic receptors and regulates
bacterial infection. Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2343-8.





Raffaele S, Bayer E, Lafarge D, Cluzet S, German Retana S, Boubekeur T,

Leborgne-Castel N, Carde JP, Lherminier J, Noirot E, Satiat-Jeunemaître B,

Laroche-Traineau J, Moreau P, Ott T, Maule AJ, Reymond P, Simon-Plas F,
Farmer

EE, Bessoule JJ, Mongrand S. Remorin, a solanaceae protein resident in
membrane

rafts and plasmodesmata, impairs potato virus X movement. Plant Cell. 2009

May;21(5):1541-55





Roche Y, Gerbeau-Pissot P, Buhot B, Thomas D, Bonneau L, Gresti J,
Mongrand S,

Perrier-Cornet JM, Simon-Plas F. Depletion of phytosterols from the plant
plasma

membrane provides evidence for disruption of lipid rafts. FASEB J. 2008

Nov;22(11):3980-91





Lin SS, Martin R, Mongrand S, Vandenabeele S, Chen KC, Jang IC, Chua NH.

RING1 E3 ligase localizes to plasma membrane lipid rafts to trigger
FB1-induced

programmed cell death in Arabidopsis. Plant J. 2008 Nov;56(4):550-61. Epub
2008

Jul 16. PubMed PMID: 18643987.







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Alicia Zelada PhD

Investigator CONICET- Assistant Professor UBA

Lab of Agrobiotechnology
FCEN-UBA

Int Güiraldes 2620
Ciudad Universitaria, Pab II. 2to piso
Facultad de Ciencias Exactas y Naturales
Universidad de Buenos Aires
1428 Buenos Aires, Argentina
Tel. (54 11) 4576-3300 ext. 425

azelada.uba en gmail.com

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